T7 RNA Polymerase CAS 9014-24-8

Identification

CAS Number

9014-24-8

EC Number

2.7.7.6

Name

T7 RNA Polymerase

EINECS

232-756-4

Properties

Optimal reaction temperature

37℃

Definition of active unit

Definition of active unit: 1 active unit is defined as the amount of enzyme needed to incorporate 1 nmol of [3H] GMP into acid-insoluble precipitate within 1h under the conditions of 37℃ and pH8.

Safety Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Specifications and Other Information of Our T7 RNA Polymerase CAS 9014-24-8

Product Introduction

This product is the phage T7 RNA Polymerase derived from recombinant expression in E. coli. It can specifically recognize the T7 promoter sequence, and synthesize RNA complementary to downstream single-stranded DNA of the promoter using the single or double stranded DNA containing the sequence of T7 promoter as the template and NTP as the substrate.

Product Features

Excellent RNA output rate; homogeneous transcript length; transcript length up to 10K.

Product Features

Product Composition

Item No.CompositionStorage Temperature
(℃ )
5KU50KU
IT7RNA Polymerase (50 U/μL)-20100μL1000μL
Buffer-20400mL4000mL

1× Buffer contains: 40 mM Tris HCl(pH7.9), 6mM MgCl2, 1mM DTT and 2mM spermidine, 10mM NaCl.

Quality Control

Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.

This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).