T7 RNA Polymerase CAS 9014-24-8
Identification
CAS Number
9014-24-8
EC Number
2.7.7.6
Name
T7 RNA Polymerase
EINECS
232-756-4
Properties
Optimal reaction temperature
37℃
Definition of active unit
Definition of active unit: 1 active unit is defined as the amount of enzyme needed to incorporate 1 nmol of [3H] GMP into acid-insoluble precipitate within 1h under the conditions of 37℃ and pH8.
Safety Data
RIDADR
NONH for all modes of transport
WGK Germany
3
Specifications and Other Information of Our T7 RNA Polymerase CAS 9014-24-8
Product Introduction
This product is the phage T7 RNA Polymerase derived from recombinant expression in E. coli. It can specifically recognize the T7 promoter sequence, and synthesize RNA complementary to downstream single-stranded DNA of the promoter using the single or double stranded DNA containing the sequence of T7 promoter as the template and NTP as the substrate.
Product Features
Excellent RNA output rate; homogeneous transcript length; transcript length up to 10K.
Product Composition
| Item No. | Composition | Storage Temperature (℃ ) | 5KU | 50KU |
| I | T7RNA Polymerase (50 U/μL) | -20 | 100μL | 1000μL |
| Ⅱ | Buffer | -20 | 400mL | 4000mL |
1× Buffer contains: 40 mM Tris HCl(pH7.9), 6mM MgCl2, 1mM DTT and 2mM spermidine, 10mM NaCl.
Quality Control
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
Links
This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).
