T4 RNA ligase CAS UENA-0195
Identification
CAS Number
UENA-0195
EC Number
6.5.1.3
Name
T4 RNA ligase
Properties
Optimal reaction temperature
37℃
Definition of active unit
Definition of active unit: 1 active unit is defined as the amount of enzyme needed to convert 1nM 5’-[32P]rA16 into anti-phosphoric acid form in 30min at 37℃ .
Safety Data
RIDADR
NONH for all modes of transport
WGK Germany
3
Specifications and Other Information of Our T4 RNA ligase CAS UENA-0195
Product Introduction
This product is the ATP-dependent T4RNA Ligase I recombinantly expressed in E. coli. It can catalyze oligonucleotide, single-stranded RNA and DNA intermolecular/ intramolecular 5’-PO4 and 3’-OH to form phosphodiester bond.
Product Composition
| Item No. | Composition | Storage Temperature (℃ ) | 1KU | 10KU |
| I | T4 RNA Ligase (10U/μL)) | -20 | 0.1mL | 1mL |
| Ⅱ | 10× Reaction Buffer | -20 | 1.5mL | 15mL |
| Ⅲ | AdENosine-5′-Triphosphate (ATP 10mM) | -20 | 0.2mL | 2mL |
| Ⅳ | PEG 8000 | -20 | 1mL | 10mL |
1x Reaction buffer contains 50mM Tris-HCL (pH7.5), 10mM MgCl2 and 1mM DTT.
Quality Control
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
Product Usage
Product Usage
• RNA 3′ terminus labelling (using cytidine-3′,5′-[-32P] diphosphate)
• Connecting RNAs
• Synthesizing oligoribonucleotide and oligodeoxyribonucleotide
• Specific modification of tRNA
• Connecting oligodeoxyribonucleotide to single-stranded cDNA to realize 5’RACE (rapid amplification of cDNA termini)
• Site-specific generation of multiplex PCR primer
Links
This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).
