- Shipping, Reconstitution & Storage
Recombinant SARS-COV-2 Spike S1 (D614G) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Gln14-Arg683(D614G).
Spike S1 (D614G)
S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.
Immobilized SARS-CoV-2 S1 (D614G) , His Tag at 2ug/ml (100ul/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 23.7ng/ml determined by ELISA. See testing image for detail.
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Shipped at ambient temperature.
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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