SARS-COV-2 Spike RBD (N501Y,K417N,E484K) Protein, Accession: QHD43416

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  • Overview
  • Specifications
  • Shipping, Reconstitution & Storage
  • Links

    • Overview

    General Description

    Recombinant SARS-COV-2 Spike RBD (N501Y,K417N,E484K) Protein is expressed from HEK293 with His tag at the C-terminal.It contains Arg319-Phe541(N501Y,K417N,E484K).

    Biotinylated

    Specifications

    Target

    Spike RBD (N501Y,K417N,E484K)

    Synonyms

    S protein RBD; S glycoprotein RBD; Spike protein RBD

    Accession

    QHD43416

    Species

    SARS-COV-2

    Exact Sequence

    Arg319-Phe541(N501Y,K417N,E484K)

    Molecular Weight

    The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.

    Activity

    Immobilized SARS-COV-2 Spike RBD (N501Y,K417N,E484K) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.

    Product Tag

    C-His

    Express System

    HEK293

    Purity

    > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

    Endotoxin

    Less than 1EU per ug by the LAL method.

    Shipping, Reconstitution & Storage

    Appearance

    Lyophilized

    Shipping Conditions

    Shipped at ambient temperature.

    Formulation

    Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

    Reconstitution

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

    Stability & Storage

    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

    This product is branded by ChemWhat and sold through our website and here is the corresponding link ChemWhat Official Website.

    This product is sold exclusively through our sales company Watson International Limited (Ltd), and please click the link below for details. (https://www.watson-int.com/), and here is the corresponding link https://www.watson-int.com/SARS-COV-2-Spike-RBD-(N501Y,K417N,E484K)-Protein-accession-QHD43416/.