RNase III CAS UENA-0194
Identification
CAS Number
UENA-0194
EC Number
3.1.26.3
Name
RNase III
Properties
Optimal reaction temperature
37℃
Definition of active unit
Definition of active unit: 1 active unit is defined as the amount of enzyme needed to degrade 1μg of dsRNA into siRNA in 20min at 37℃ in a 50μL reaction system.
Safety Data
RIDADR
NONH for all modes of transport
WGK Germany
3
Specifications and Other Information of Our RNase III CAS UENA-0194
Product Introduction
This product is the RNase Ⅲ recombinantly expressed in E. coli. This specific exonuclease is capable of cutting double-stranded RNA (dsRNA) and generating 12-35bp dsRNA fragments with protruding 5′-PO4, 3′-OH, and 3’ termini.
Product Features
Efficient production of siRNA; accurate interference with target gene expression.
Product Composition
| Item No. | Composition | Storage Temperature (℃ ) | 200U | 2000U |
| I | RNase III (2U/μL) | -20 | 0.5mL | 5mL |
| Ⅱ | 10x Reaction Buffer | -20 | 1.5mL | 15mL |
| Ⅲ | 10x EDTA | -20 | 1mL | 10mL |
| Ⅳ | 10x MnCl2 | -20 | 1mL | 10mL |
10× Reaction buffer contains 500mM Tris-HCl(pH7.5)500mM NaCl and 10mM DTT.
Quality Control
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
Links
This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).