Recombinant trypsin EC 18.104.22.168 CAS 9002-07-7
Trypsin-EDTA Solution 1X;
Trypsin Powder, Porcine 1:250;
TRYPSIN TYPE V-S:
ACETYLATED FROM*BOVINE PANCREAS;
TRYPSIN, PROTEOMICS SEQUENCING GRADE;
White or off white, or yellowish powder
Hazard statementsH315 – H319 – H334 – H335
Precautionary StatementsP302 + P352 – P305 + P351 + P338
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
NONH for all modes of transport
Specifications and Other Information of Our Recombinant trypsin EC 22.214.171.124 CAS 9002-07-7
|Items||Recombinant Trypsin||Recombinant Trypsin (Liquid)||Recombinant Human Trypsin||Sequencing Grade Modified Trypsin|
|Appearance||White or off white, or yellowish powder||Clear, colorless or shallow yellow liquid||White or off white powder||White or off white, or yellowish powder|
|Protein Concentration||/||70±10 mg/ml||/||/|
|Specific activity||≥3800 USP units/mg pro.||≥3800 (U/mg protein)||≥2500 USP units/mg pro.||≥4500 USP units/mg pro.|
|E. Coli host Cell protein residue||/||≤0.01%||/||/|
|Host Cell DNA residues||/||≤ 10 ng/mg||/||/|
|Purity(SDS-PAGE)||Single major band||/||/||Single major band|
|Molecular Weight(SDS-PAGE)||24.0±2.4 kDa||/||/||24.0±2.4 kDa|
|Unit definition||One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).||One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).||/||/|
|Activity unit||25℃, pH7.6, 3.2 ml reaction solution(1 cm light path), one trypsin unit (USP) was defined as an increase of 0.003 in the absorption value at 253 nm by enzymatic hydrolysis of BAEE per minute.|
|Recommended usage||The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.||Prepare 1-10mg/ml recombinant human trypsin .The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.||It is recommended to dissolve or dilute with 50 mM HAc. When used, dilute in 50 mM NH4HCO3 or ph7.0-8.0 buffer solution. 1 mM CaCl2 is recommended In the digestion buffer. The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-8.0.|
|Stability of storage and transport||Recombinant trypsin lyophilized should be stored under 2-8℃ in sealed container.|
It is stable within 24 months.After dissolved wth 50mM HAC, it should be stored under -20℃. It is no activity loss after 10 times repeated freezing and thawing.
The product is stable by bue ice insulation transport.
|The recombinant trypsin solution was stored under -20℃ and was stable for 12 months;|
Dry ice insulation transportation, won’t melt during transportation, stable activity.
|Recombinant human trypsin lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.It is no activity loss after 10 times repeated freezing and thawing.|
The product is stable by blue ice insulation transport.
|Sequencing grade trypsin lyophilized should be stored under 2-8℃ in sealed container.It is stable within 24 months.After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20℃. It is no activity loss after 5 times repeated freezing and thawing.|
The product is stable by blue ice insulation transport.
|Usage||The amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin .Recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes,such as in insulin and vaccine production.||For cell culture:|
Tissue block digestion, primary cell acquisition;
Passage digestion of adherent cells;
Cell culture by microcarrier method;
Gently digesting stem cells;
Immune cell therapy, etc.
For recombinant protein:
Recombinant insulin production;
Protein sequencing, peptide mapping;
Specific proteolytic processes such as proteomics research.
|Recombinant human trypsin is an endopeptidase that can be used for lysine and arginine C-terminal cleavage of peptide bonds to cleave macromolecules into small peptides. Recombinant human trypsin is widely used in a variety of biotechnological processes, such as: cell culture cell separation of various tissues; degradation of denatured proteins; enzymatic hydrolysis, sequencing; stem cell therapy; tumor cell therapy.||The amino acid sequence of sequencing grade recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin, and there are many advantages, such as no enzyme activity, high stability, non-self-cutting, high activity.When used, it is no non-specific fragments and self-cutting fragments.Sequencing grade recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes,such as specific protein digestion, protein sequencing, peptide mapping, proteomics studies, Z-D peptides on the peptidase digestion.|
|Source||Recombinant trypsin is a genetically engineered protein expressed in E.coli||Recombinant Trypsin (Porcine), produced by genetic engineering, have the same amino acid sequence as the porcine pancreas trypsin.||Recombinant human trypsin is a genetically engineered protein expressed in E.coli||Sequencing grade modified trypsin is a genetically engineered methylation modified protein expressed in E.coli|
- Animal origin free: recombinant trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
- Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
- High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
- Lyophilized powder: The product is lyophilized powder and is easy to store and transport.