Recombinant Soybean Trypsin Inhibitor CAS 9035-81-8
Identification
CAS Number
9035-81-8
Name
Recombinant Soybean Trypsin Inhibitor
Synonyms
iniprol;
SBTI/STI;
contrykal;
APROTINIUM;
Ovomucoid-Agarose;
EZNA KIT MICRO RNA;
Trypsin Inhibitor So;
KUNITZ TRYPSIN INHIBITOR;
TRYPSIN INHIBITOR CHICKEN;
TrypsinInhibitorExLimaBean
EINECS
232-906-9
MDL Number
MFCD00082103
Properties
Appearance
white, off-white, off-yellow powder
Safety Data
Personal Protective Equipment
Eyeshields, Gloves, type N95 (US)
RIDADR
NONH for all modes of transport
WGK Germany
3
Specifications and Other Information of Our Recombinant Soybean Trypsin Inhibitor CAS 9035-81-8
Purity(SDS-PAGE)
≥90%
Specific activity
≥1.0 U / mg pro
Protein content
90% -110% of the indicated amount
Electrophoresis identification (SDS-PAGE)
a single main band
The relative molecular weight
20.1±2.0 kD
Inhibitor enzyme activity unit
The ability to inhibit the activity of a trypsin unit is called an inhibitor activity unit (U).
Trypsin unit
One trypsin unit (micro katal) is defined to hydrolyze 1 μmol of N-benzoyl-L-arginine ethyl ester (BAEE) per second.
Introduction
Soybean trypsin inhibitor is a serine protease inhibitor, which can inhibit the activity of trypsin, chymotrypsin, and plasmin. The ability to inhibit trypsin is higher than that of chymotrypsin and plasmin. In addition, it can inhibit plasma kallikrein, clotting factor Xa, but can’t inhibit metalloproteinase, tissue kallikreins, acid protease, and sulfur-containing protease. Inhibitors and protease can be effectively combined in equimolar amounts to form stable complexes to block the active site of the enzyme. A suitable pH range for inhibiting trypsin is pH 6.0-10.0. Recombinant soybean trypsin inhibitor was expressed by recombinant E.coli and purified without animal-derived contamination and no other protease.
Instruction
Inhibitors and protease can be combined in equimolar amounts, the recommended pH for combination is pH>6.0 , inhibitors and protease won’t be combined when pH<5.0.
Source
Produced by genetic engineering, expressed in E.coli.
Stability of storage
Lyophilized powder is stable for 24 months at 2-8℃
Stability of transport
Blue ice insulation transportation, stable activity.
Advantage
1.Animal origin free: The product is produced by recombination and no any animal source material is used in the production process. No exogenous virus contamination, safe to use.
2.Stable quality: Stable and continuous batch supply, little difference between batches of products, stable quality.
Known Application
Soybean trypsin inhibitor is a serine protease inhibitor. It can form a stable complex with protease to block the active site of the enzyme. Such as: inhibition of serine protease activity in recombinant protein production; cell culture and other biotechnology processes.
