Recombinant Rat Tumor Necrosis Factor-alpha/TNFSF2 (rRtTNF-alpha/TNFSF2)

Official Full Name

Recombinant Rat Tumor Necrosis Factor-alpha/TNFSF2 (rRtTNF-alpha/TNFSF2)

Synonyms

Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing Factor , DIF, Necrosin, Cytotoxin

Squence

Squence of Recombinant Rat Tumor Necrosis Factor-alpha/TNFSF2

Amino Acid Sequence

LRSSSQNSSD KPVAHVVANH QAEEQLEWLS QRANALLANG MDLKDNQLVV PADGLYLIYS QVLFKGQGCP DYVLLTHTVS RFAISYQEKV SLLSAIKSPC PKDTPEGAEL KPWYEPMYLG GVFQLEKGDL LSAEVNLPKY LDITESGQVY FGVIAL

Synonyms

Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing Factor , DIF, Necrosin, Cytotoxin

Accession

P16599

Gene ID

24835

Summary

Tumor necrosis factor alpha (TNF-alpha), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-alpha occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-alpha is glycosylated, but non-glycosylated recombinant TNF-alpha has comparable biological activity. Two types of receptors for TNF-alpha have been described and virtually all cell types studied show the presence of one or both of these receptor types.

Source

Escherichia coli.

Molecular Weight

Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg in the presence of actinomycin D.

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.2, 150 mM NaCl.

Endotoxin

Less than 1 EU/ug of rRtTNF-α as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Purity

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE

SDS-PAGE of Recombinant Rat Tumor Necrosis Factor-alpha/TNFSF2

Pack Size

5ug/100ug/500ug

Safety Data Sheet (SDS) Download

Click to download

Technical Data Sheet (TDS) Download

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Links

This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Rat Tumor Necrosis Factor-alpha/TNFSF2 (rRtTNF-alpha/TNFSF2) on ChemWhat