Recombinant Rat Macrophage Inflammatory Protein-1 beta/CCL4 (rRtMIP-1beta/CCL4)
Official Full Name
Recombinant Rat Macrophage Inflammatory Protein-1 beta/CCL4 (rRtMIP-1beta/CCL4)
Synonyms
Small-inducible Cytokine A4
Squence
Amino Acid Sequence
APIGSDPPTS CCFSYTSRKI HRNFVMDYYE TSSLCSQPAV VFLTKKGRQI CADPSEPWVN EYVNDLELN
Synonyms
Small-inducible Cytokine A4
Accession
Gene ID
Summary
Source
Escherichia coli.
Molecular Weight
Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.
Biological Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-1000 ng/ml.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in 2 × PBS, pH 7.4, 3 % trehalose.
Endotoxin
Less than 1 EU/ug of rRtMIP-1β/CCL4 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
1. Miyamoto MNaruo K, Seko C, et al. 1993. Mol Cell Biol. 13:4251-9.2. Santos-Ocampo S, Colvin JS, Chellaiah A, et al. 1996. J Biol Chem. 271:1726-31.3. Chellaiah A, Yuan W, Chellaiah M, et al. 1999. J Biol Chem. 274:34785-94.
Purity
> 95 % by SDS-PAGE and HPLC analyses.
SDS-PAGE
Pack Size
5ug/100ug/500ug
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Rat Macrophage Inflammatory Protein-1 beta/CCL4 (rRtMIP-1beta/CCL4) on ChemWhat
