Recombinant Rat Macrophage Inflammatory Protein-1 beta/CCL4 (rRtMIP-1beta/CCL4)

Official Full Name

Recombinant Rat Macrophage Inflammatory Protein-1 beta/CCL4 (rRtMIP-1beta/CCL4)

Synonyms

Small-inducible Cytokine A4

Squence

Squence of Recombinant Rat Macrophage Inflammatory Protein-1 beta/CCL4

Amino Acid Sequence

APIGSDPPTS CCFSYTSRKI HRNFVMDYYE TSSLCSQPAV VFLTKKGRQI CADPSEPWVN EYVNDLELN

Synonyms

Small-inducible Cytokine A4

Accession

P50230

Gene ID

116637

Summary

Source

Escherichia coli.

Molecular Weight

Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-1000 ng/ml.

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered concentrated solution in 2 × PBS, pH 7.4, 3 % trehalose.

Endotoxin

Less than 1 EU/ug of rRtMIP-1β/CCL4 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

References

1. Miyamoto MNaruo K, Seko C, et al. 1993. Mol Cell Biol. 13:4251-9.2. Santos-Ocampo S, Colvin JS, Chellaiah A, et al. 1996. J Biol Chem. 271:1726-31.3. Chellaiah A, Yuan W, Chellaiah M, et al. 1999. J Biol Chem. 274:34785-94.

Purity

> 95 % by SDS-PAGE and HPLC analyses.

SDS-PAGE

SDS-PAGE of Recombinant Rat Macrophage Inflammatory Protein-1 beta/CCL4

Pack Size

5ug/100ug/500ug

Safety Data Sheet (SDS) Download

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Technical Data Sheet (TDS) Download

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Links

This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Rat Macrophage Inflammatory Protein-1 beta/CCL4 (rRtMIP-1beta/CCL4) on ChemWhat