Recombinant Rat Interleukin-6 (rRtIL-6)
Official Full Name
Recombinant Rat Interleukin-6 (rRtIL-6)
Synonyms
Squence
Amino Acid Sequence
FPTSQVRRGD FTEDTTHNRP VYTTSQVGGL ITYVLREILE MRKELCNGNS DCMNSDDALS ENNLKLPEIQ RNDGCFQTGY NQEICLLKIC SGLLEFRFYL EFVKNNLQDN KKDKARVIQS NTETLVHIFK QEIKDSYKIV LPTPTSNALL MEKLESQKEW LRTKTIQLIL KALEEFLKVT MRSTRQT
Synonyms
Accession
Gene ID
Summary
Source
Escherichia coli.
Molecular Weight
Approximately 21.7 kDa, a single, non-glycosylated polypeptide chain containing 187 amino acids
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 × 108 IU/mg.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Endotoxin
Less than 1 EU/ug of rRtIL-6 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HCl to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
1. Ferguson-Smith AC, Chen YF, Newman MS, et al. 1988. Genomics. 2:203-8.2. van der Poll T, Keogh CV, Guirao X, et al. 1997. J Infect Dis. 176:439-44.3. Bastard JP, Jardel C, Delattre J, et al. 1999. Circulation. 99:2221-2.4. Northemann W, Braciak TA, Hattori M, et al. 1989. J Biol Chem. 264:16072-82.5. Heinrich PC, Behrmann I, Muller-Newen G, et al. 1998. Biochem J. 334 ( Pt 2):297-314.6. Van Snick J, Cayphas S, Szikora JP, et al. 1988. Eur J Immunol. 18:193-7.
Purity
> 95 % by SDS-PAGE and HPLC analyses.
SDS-PAGE
Pack Size
10ug/100ug/500ug
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Rat Interleukin-6 (rRtIL-6) on ChemWhat
