Recombinant Rat Granulocyte-Macrophage Colony Stimulating Factor (rRtGM-CSF)
Official Full Name
Recombinant Rat Granulocyte-Macrophage Colony Stimulating Factor (rRtGM-CSF)
Synonyms
Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-α
Squence
Amino Acid Sequence
APTRSPNPVT RPWKHVDAIK EALSLLNDMR ALENEKNEDV DIISNEFSIQ RPTCVQTRLK LYKQGLRGNL TKLNGALTMI ASHYQTNCPP TPETDCEIEV TTFEDFIKNL KGFLFDIPFD CWKPVQK
Synonyms
Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-α
Accession
Gene ID
Summary
Source
Escherichia coli.
Molecular Weight
Approximately 14.5 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine FDC-P1 is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 × 108 IU/mg.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Endotoxin
Less than 1 EU/ug of rRtGM-CSF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
1. Wang JM, Chen ZG, Colotta F, et al. 1988. Behring Inst Mitt: 270-3.2. 1989. N Engl J Med, 320: 253-4.3. Nissen-Druey C. 1989. Nouv Rev Fr Hematol, 31: 99-101.4. Eager RandNemunaitis J. 2005. Mol Ther, 12: 18-27.5. Tran T, Fernandes DJ, Schuliga M, et al. 2005. Br J Pharmacol, 145: 123-31.
Purity
> 98 % by SDS-PAGE and HPLC analyses.
SDS-PAGE
Pack Size
5ug/100ug/500ug
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Rat Granulocyte-Macrophage Colony Stimulating Factor (rRtGM-CSF) on ChemWhat
