Recombinant Rat Glial Cell-derived Neurotrophic Factor (rRtGDNF)
Official Full Name
Recombinant Rat Glial Cell-derived Neurotrophic Factor (rRtGDNF)
Synonyms
ATF
Squence
Amino Acid Sequence
SPDKQAAALP RRERNRQAAA ASPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCEAAETMYD KILKNLSRSR RLTSDKVGQA CCRPVAFDDD LSFLDDSLVY HILRKHSAKR CGCI
Synonyms
ATF
Accession
Gene ID
Summary
Source
Escherichia coli.
Molecular Weight
Approximately 29.8 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in 1 × PBS, pH 7.4.
Endotoxin
Less than 0.1 EU/ug of rRtGDNF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
1. Lin LF, Doherty DH, Lile JD, et al. 1993. Science. 260:1130-2.2. Russell FD, Koishi K, Jiang Y, et al. 2000. Neuroscience. 97:575-80.3. Jing S, Yu Y, Fang M, et al. 1997. J Biol Chem. 272:33111-7.4. Cik M, Masure S, Lesage AS, et al. 2000. J Biol Chem. 275:27505-12.
Purity
> 98 % by SDS-PAGE and HPLC analyses.
SDS-PAGE
Pack Size
10ug/100ug/500ug
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Rat Glial Cell-derived Neurotrophic Factor (rRtGDNF) on ChemWhat
