Recombinant Rat Cytokine-induced Neutrophil Chemoattractant 3/CXCL2 (rRtCINC-3/CXCL2)

Official Full Name

Recombinant Rat Cytokine-induced Neutrophil Chemoattractant 3/CXCL2 (rRtCINC-3/CXCL2)

Synonyms

MIP-2

Squence

Squence of Recombinant Rat Cytokine-induced Neutrophil Chemoattractant 3/CXCL2

Amino Acid Sequence

VVVASELRCQ CLTTLPRVDF KNIQSLTVTP PGPHCAQTEV IATLKDGHEV CLNPEAPLVQ RIVQKILNKG KAN

Synonyms

MIP-2

Accession

P30348

Gene ID

114105

Summary

Source

Escherichia coli.

Molecular Weight

Approximately 7.9 kDa, a single, non-glycosylated polypeptide chain containing 73 amino acids.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using rat neutrophils is in a concentration range of 10-100 ng/ml.

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.

Endotoxin

Less than 1 EU/ug of rRtCINC-3/CXCL2 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

References

1. Haskill S, Peace A, Morris J, et al. 1990. Proc Natl Acad Sci U S A. 87:7732-6.2. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83.3. Wolpe SD, Sherry B, Juers D, et al. 1989. Proc Natl Acad Sci U S A. 86:612-6.4. Iida N, Grotendorst GR. 1990. Mol Cell Biol. 10:5596-9.5. Pelus LM, Fukuda S. 2006. Exp Hematol. 34:1010-20.

Purity

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE

SDS-PAGE of Recombinant Rat Cytokine-induced Neutrophil Chemoattractant 3/CXCL2

Pack Size

5ug/100ug/500ug

Safety Data Sheet (SDS) Download

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Technical Data Sheet (TDS) Download

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Links

This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Rat Cytokine-induced Neutrophil Chemoattractant 3/CXCL2 (rRtCINC-3/CXCL2) on ChemWhat