Recombinant Rat Cytokine-induced Neutrophil Chemoattractant-2 beta/CXCL3 (rRtCINC-2beta/CXCL3)

Official Full Name

Recombinant Rat Cytokine-induced Neutrophil Chemoattractant-2 beta/CXCL3 (rRtCINC-2beta/CXCL3)

Synonyms

CINC-2beta, MIP2-beta

Squence

Squence of Recombinant Rat Cytokine-induced Neutrophil Chemoattractant-2 beta/CXCL3

Amino Acid Sequence

RELRCQCLKT LPRVDFENIQ SLTVTPPGPH CTQTEVIATL KDGQEVCLNP QAPRLQKIIQ KLLKSPSL

Synonyms

CINC-2beta, MIP2-beta

Accession

Q10746

Gene ID

171551

Summary

Source

Escherichia coli.

Molecular Weight

Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 5-50 ng/ml.

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.

Endotoxin

Less than 1 EU/ug of rRtCINC-2β/CXCL3 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

References

1. Nolan KF, Strong V, Soler D, et al. 2004. J Immunol. 172:2201-9.2. Modi WS, Yoshimura T. 1999. Mol Biol Evol. 16:180-93.3. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83.4. Smith DF, Galkina E, Ley K, et al. 2005. Am J Physiol Heart Circ Physiol. 289:H1976-84.5. Nakagawa H, Komorita N, Shibata F, et al. 1994. Biochem J. 301 ( Pt 2):545-50.

Purity

> 96 % by SDS-PAGE and HPLC analyses.

SDS-PAGE

SDS-PAGE of Recombinant Rat Cytokine-induced Neutrophil Chemoattractant-2 beta/CXCL3

Pack Size

10ug/100ug/500ug

Safety Data Sheet (SDS) Download

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Technical Data Sheet (TDS) Download

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Links

This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Rat Cytokine-induced Neutrophil Chemoattractant-2 beta/CXCL3 (rRtCINC-2beta/CXCL3) on ChemWhat