Recombinant Rat Cytokine-induced Neutrophil Chemoattractant 1/CXCL1 (rRtCINC-1/CXCL1)
Official Full Name
Recombinant Rat Cytokine-induced Neutrophil Chemoattractant 1/CXCL1 (rRtCINC-1/CXCL1)
Synonyms
GRO-alpha, Platelet-derived Growth Factor-inducible Protein KC
Squence
Amino Acid Sequence
APVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREA CLDPEAPMVQ KIVQKMLKGV PK
Synonyms
GRO-alpha, Platelet-derived Growth Factor-inducible Protein KC
Accession
Gene ID
Summary
Source
Escherichia coli.
Molecular Weight
Approximately 7.8 kDa, a single, non-glycosylated polypeptide chain containing 72 amino acids.
Biological Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using rat neutrophils is in a concentration range of 10-100 ng/ml.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Endotoxin
Less than 1 EU/ug of rRtCINC-1/CXCL1 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
1. Haskill S, Peace A, Morris J, et al. 1990. Proc Natl Acad Sci U S A. 87:7732-6.2. Richmond A, Thomas HG. 1988. J Cell Biochem. 36:185-98.3. Iida N, Grotendorst GR. 1990. Mol Cell Biol. 10:5596-9.4. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83.5. Moser B, Clark-Lewis I, Zwahlen R, et al. 1990. J Exp Med. 171:1797-802.6. Devalaraja RM, Nanney LB, Du J, et al. 2000. J Invest Dermatol. 115:234-44.
Purity
> 97 % by SDS-PAGE and HPLC analyses.
SDS-PAGE
Pack Size
5ug/100ug/500ug
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Rat Cytokine-induced Neutrophil Chemoattractant 1/CXCL1 (rRtCINC-1/CXCL1) on ChemWhat
