Recombinant Proteinase K EC 3.4.21.14 CAS 39450-01-6

• Identification
• Properties
• Safety Data
• Specifications & Other Information
• Links

Identification

CAS Number

39450-01-6

EC Number

3.4.21.14

Name

Recombinant Proteinase K

Synonyms

1H-Benzimidazole-5-carboxamide, 1-(2-methoxyethyl)-N-[1-methyl-2-(1-methylethyl)-1H-benzimidazol-5-yl]- [ACD/Index Name]
N-(2-Isopropyl-1-methyl-1H-benzimidazol-5-yl)-1-(2-methoxyethyl)-1H-benzimidazol-5-carboxamid [German] [ACD/IUPAC Name]
N-(2-Isopropyl-1-methyl-1H-benzimidazol-5-yl)-1-(2-methoxyethyl)-1H-benzimidazole-5-carboxamide [ACD/IUPAC Name]
N-(2-Isopropyl-1-méthyl-1H-benzimidazol-5-yl)-1-(2-méthoxyéthyl)-1H-benzimidazole-5-carboxamide [French] [ACD/IUPAC Name]
1-(2-methoxyethyl)-N-(1-methyl-2-propan-2-ylbenzimidazol-5-yl)benzimidazole-5-carboxamide
1214393-36-8 [RN]
39450-01-6 [RN]
proteinase k, lyophilized powder, biotechgrade

SMILES

CC(C1C(=O)N2C1(C(C(=C2C(=O)OCC3=CC=C(C=C3)[N+](=O)[O-])OC4=CC=CC=C4)(C)OC5=CC=CC=C5)OP(=O)(O)O)O

InChI

InChI=1S/C29H27N2O12P/c1-18(32)23-26(33)30-24(27(34)40-17-19-13-15-20(16-14-19)31(35)36)25(41-21-9-5-3-6-10-21)28(2,29(23,30)43-44(37,38)39)42-22-11-7-4-8-12-22/h3-16,18,23,32H,17H2,1-2H3,(H2,37,38,39)/t18-,23-,28-,29+/m1/s1

InChI Key

OUPAKHMWUJJXOM-ANOMEZDXSA-N

Molecular Formula

C₂₂H₂₅N₅O₂

Molecular Weight

391.466

EINECS

254-457-8

MDL Number

MFCD00132129

Properties

Appearance

Lyophilized

Safety Data

Symbol

GHS07,GHS08

Signal Word

Danger

Hazard statements

H315 – H319 – H334 – H335

Precautionary Statements

P302 + P352 – P305 + P351 + P338

Target organs

Respiratory system

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

RIDADR 

NONH for all modes of transport

WGK Germany

1

Specifications and Other Information of Our Recombinant Proteinase K EC 3.4.21.14 CAS 39450-01-6

Purity

≥95%

Activity

≥30 units/mg

Molecular Weight(SDS-PAGE)

29.3kD

Unit definition

One unit is defined as the amount of enzyme that will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 mol tyrosine per min at 37°C, pH 7.5(color by Folin-Ciocalteu reagent).

Description

A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).

Recommended usage

Prepare recombinant proteinase K with 20mM Tris-HCl (pH 7.4~8.0),1mM CaCl₂ ,50% glycerol.The optimum pH is 8.0.

Storage

Store lyophilized at -20°C. Aliquot the product after reconstitution to avoid repeated freezing thawing cycles.

Links

Watson International Ltd

This product is sold exclusively through our sales company Watson International Ltd, and please click the link below for details. http://www.watson-int.com/recombinant-proteinase-k-ec-3-4-21-14-cas-39450-01-6/