Recombinant Porcine Interleukin-8/CXCL8 (rPoIL-8/CXCL8)

Official Full Name

Recombinant Porcine Interleukin-8/CXCL8 (rPoIL-8/CXCL8)

Synonyms

(Ser-IL-8)72, GCP/IL-8 Protein I, IL8/NAP1 form III, LYNAP, MDNCF-c, NAF

Squence

Squence of Recombinant Porcine Interleukin-8/CXCL8

Amino Acid Sequence

ARVSAELRCQ CINTHSTPFH PKFIKELRVI ESGPHCENSE IIVKLVNGKE VCLDPKEKWV QKVVQIFLKR TEKQQQQQ

Synonyms

(Ser-IL-8)72, GCP/IL-8 Protein I, IL8/NAP1 form III, LYNAP, MDNCF-c, NAF

Accession

P26894

Gene ID

396880

Summary

Interleukin-8 (IL-8) is encoded by the IL8 gene and produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. There are many receptors capable to bind IL-8, the most affinity to IL-8 are receptors CXCR1, and CXCR2. As a member of the CXC chemokine family, function of IL-8 is the induction of chemotaxis in its target cells, like neutrophil granulocytes, basophils, and T-cells. . IL-8 is often associated with inflammation and has been cited as a proinflammatory mediator in gingivitis and psoriasis.

Source

Escherichia coli.

Molecular Weight

Approximately 9.1 kDa, a single non-glycosylated polypeptide chain containing 78 amino acids.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 20-200 ng/ml.

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered concentrated solution in 2 × PBS, pH 7.4.

Endotoxin

Less than 1 EU/ug of rPoIL-8/CXCL8 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Purity

> 95 % by SDS-PAGE and HPLC analyses.

SDS-PAGE

SDS-PAGE of Recombinant Porcine Interleukin-8/CXCL8

Pack Size

10ug/100ug/500ug

Safety Data Sheet (SDS) Download

Click to download

Technical Data Sheet (TDS) Download

Click to download

Links

This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Porcine Interleukin-8/CXCL8 (rPoIL-8/CXCL8) on ChemWhat