Recombinant Murine Vascular Endothelial Growth Factor 164 (rMuVEGF164)
Official Full Name
Recombinant Murine Vascular Endothelial Growth Factor 164 (rMuVEGF164)
Synonyms
Vascular Endothelial Growth Factor Isoform 164
Squence
Amino Acid Sequence
MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Synonyms
Vascular Endothelial Growth Factor Isoform 164
Accession
Gene ID
Summary
Source
Escherichia coli.
Molecular Weight
Approximately 38.8 kDa, a disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains with Met at N-terminus.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Endotoxin
Less than 1 EU/ug of rMuVEGF164 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
1. Leung DW, Cachianes G, Kuang WJ, et al. 1989. Science. 246:1306-9.2. Byrne AM, Bouchier-Hayes DJ, Harmey JH. 2005. J Cell Mol Med. 9:777-94.3. Robinson CJ, Stringer SE. 2001. J Cell Sci. 114:853-65.
Purity
> 95 % by SDS-PAGE and HPLC analyses.
SDS-PAGE
Pack Size
10ug/100ug/500ug
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Murine Vascular Endothelial Growth Factor 164 (rMuVEGF164) on ChemWhat
