Recombinant Murine Thymus Expressed Chemokine/CCL25 (rMuTECK/CCL25)

Official Full Name

Recombinant Murine Thymus Expressed Chemokine/CCL25 (rMuTECK/CCL25)

Synonyms

SCYA25, Ckb15

Squence

Squence of Recombinant Murine Thymus Expressed Chemokine/CCL25

Amino Acid Sequence

QGAFEDCCLG YQHRIKWNVL RHARNYHQQE VSGSCNLRAV RFYFRQKVVC GNPEDMNVKR AIRILTARKR LVHWKSASDS QTERKKSNHM KSKVENPNST SVRSATLGHP RMVMMPRKTN N

Synonyms

SCYA25, Ckb15

Accession

O35903

Gene ID

Summary

CCL25 is new member of CC family chemokine. It is also called Thymus-expressed chemokine (TECK) because it is restricted produced by thymus and intestine. Especially, the dendritic cells derived from thymus but not bone marrow had identified to be the source of CCL25. By binding with CCR9, it elicits its effects of chemotactic for thymocytes, macrophages, and dendritic cells. Additionally, CCL25 takes part in regulating the development of T-cells.

Source

Escherichia coli.

Molecular Weight

Approximately 14.1 kDa, a single, non-glycosylated polypeptide chain containing 121 amino acids.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 5.0-50 ng/ml.

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Endotoxin

Less than 1 EU/ug of rMuTECK/CCL25 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Purity

> 95 % by SDS-PAGE and HPLC analyses.

SDS-PAGE

SDS-PAGE of Recombinant Murine Thymus Expressed Chemokine/CCL25

Pack Size

5ug/100ug/500ug

Safety Data Sheet (SDS) Download

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Technical Data Sheet (TDS) Download

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Links

This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Murine Thymus Expressed Chemokine/CCL25 (rMuTECK/CCL25) on ChemWhat