Recombinant Murine Stromal-Cell Derived Factor-1 beta/CXCL12β (rMuSDF-1beta/CXCL12beta)

Official Full Name

Recombinant Murine Stromal-Cell Derived Factor-1 beta/CXCL12β (rMuSDF-1beta/CXCL12beta)

Synonyms

TPAR1, PBSF, TLSF

Squence

Squence of Recombinant Murine Stromal-Cell Derived Factor-1 beta/CXCL12β

Amino Acid Sequence

KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNKRL KM

Synonyms

TPAR1, PBSF, TLSF

Accession

P40224

Gene ID

20315

Summary

Source

Escherichia coli.

Molecular Weight

Approximately 8.5 kDa, a single non-glycosylated polypeptide chain containing 72 amino acids.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/ml.

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Endotoxin

Less than 1 EU/ug of rMuSDF-1β/CXCL12βas determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

References

1. Shirozu M, Nakano T, Inazawa J, et al. 1995. Genomics. 28:495-500.2. De La Luz Sierra M, Yang F, Narazaki M, et al. 2004. Blood. 103:2452-9.3. Charnaux N, Brule S, Hamon M, et al. 2005. FEBS J. 272:1937-51.4. Bleul CC, Fuhlbrigge RC, Casasnovas JM, et al. 1996. J Exp Med. 184:1101-9.5. Ara T, Nakamura Y, Egawa T, et al. 2003. Proc Natl Acad Sci U S A. 100:5319-23.6. Askari AT, Unzek S, Popovic ZB, et al. 2003. Lancet. 362:697-703.7. Ma Q, Jones D, Borghesani PR, et al. 1998. Proc Natl Acad Sci U S A. 95:9448-53.

Purity

> 97 % by SDS-PAGE and HPLC analyses.

SDS-PAGE

SDS-PAGE of Recombinant Murine Stromal-Cell Derived Factor-1 beta/CXCL12β

Pack Size

10ug/100ug/500ug

Safety Data Sheet (SDS) Download

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Technical Data Sheet (TDS) Download

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Links

This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Murine Stromal-Cell Derived Factor-1 beta/CXCL12β (rMuSDF-1beta/CXCL12beta) on ChemWhat