Recombinant Murine Monocyte Chemotactic Protein-5/CCL12 (rMuMCP-5/CCL12)
Official Full Name
Recombinant Murine Monocyte Chemotactic Protein-5/CCL12 (rMuMCP-5/CCL12)
Synonyms
MCP-1-related Chemokine, Small-inducible Cytokine A12
Squence
Amino Acid Sequence
GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG
Synonyms
MCP-1-related Chemokine, Small-inducible Cytokine A12
Accession
Gene ID
Summary
Source
Escherichia coli.
Molecular Weight
Approximately 9.3 kDa, a single non-glycosylated polypeptide chain containing 82 amino acids.
Biological Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-50 ng/ml.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Endotoxin
Less than 1 EU/ug of rMuMCP-5/CCL12 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
1. Sarafi MN, Garcia-Zepeda EA, MacLean JA, et al. 1997. J Exp Med. 185:99-109.2. Jia GQ, Gonzalo JA, Lloyd C, et al. 1996. J Exp Med. 184:1939-51.
Purity
> 95 % by SDS-PAGE and HPLC analyses.
SDS-PAGE
Pack Size
5ug/100ug/500ug
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Murine Monocyte Chemotactic Protein-5/CCL12 (rMuMCP-5/CCL12) on ChemWhat
