Recombinant Murine Macrophage Inflammatory Protein-3 beta/CCL19 (rMuMIP-3beta/CCL19)
Official Full Name
Recombinant Murine Macrophage Inflammatory Protein-3 beta/CCL19 (rMuMIP-3beta/CCL19)
Synonyms
Beta-chemokine Dxodus-3, CK beta-11, EBI1 Ligand Dhemokine, ELCMacrophage Inflammatory Protein 3 beta
Squence
Amino Acid Sequence
GANDAEDCCL SVTQRPIPGN IVKAFRYLLN EDGCRVPAVV FTTLRGYQLC APPDQPWVDR IIRRLKKSSA KNKGNSTRRS PVS
Synonyms
Beta-chemokine Dxodus-3, CK beta-11, EBI1 Ligand Dhemokine, ELCMacrophage Inflammatory Protein 3 beta
Accession
Gene ID
Summary
CCL19, also known as EBI1 Ligands chemokine (ELC) and macrophage inflammatory protein-3-beta, is encoded by the CCL19 gene which is located on chromosome 4 in mouse. The protein is expressed at high levels in thymus and lymph nodes, with intermediate levels in trachea and colon and low levels in stomach, lung and some other organs. CCL19 is signal through CCR7 and chemotaxis of T and B lymphocytes and myeloid progenitor cells.
Source
Escherichia coli.
Molecular Weight
Approximately 9.2 kDa, a single non-glycosylated polypeptide chain containing 83 amino acids.
Biological Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human mature dendritic cells is in a concentration range of 10-100 ng/ml.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Endotoxin
Less than 1 EU/ug of rMuMIP-3β/CCL19 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
Purity
> 97 % by SDS-PAGE and HPLC analyses.
SDS-PAGE
Pack Size
5ug/100ug/500ug
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Murine Macrophage Inflammatory Protein-3 beta/CCL19 (rMuMIP-3beta/CCL19) on ChemWhat
