Recombinant Murine Macrophage Inflammatory Protein-1 beta/CCL4 (rMuMIP-1beta/CCL4)
Official Full Name
Recombinant Murine Macrophage Inflammatory Protein-1 beta/CCL4 (rMuMIP-1beta/CCL4)
Synonyms
ACT-2, MIP-1-beta, Protein H400, SIS-gamma, Small-inducible cytokine A4
Squence
Amino Acid Sequence
APMGSDPPTS CCFSYTSRQL HRSFVMDYYE TSSLCSKPAV VFLTKRGRQI CANPSEPWVT EYMSDLELN
Synonyms
ACT-2, MIP-1-beta, Protein H400, SIS-gamma, Small-inducible cytokine A4
Accession
Gene ID
Summary
Chemokine (C-C motif) ligand 4, also known as Macrophage inflammatory protein-1β (MIP-1β) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. In addition, it is a monokine with inflammatory and chemokinetic properties. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Furthermore, recombinant murine CCL4 contains 69 amino acids and it shares 77 % and 86 % a.a. sequence identity with human and rat CCL4. Both human and murine MIP-1alpha and MIP-1β are active on human and murine hematopoietic cells.
Source
Escherichia coli.
Molecular Weight
Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.
Biological Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 20-100 ng/ml.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in 2 × PBS, pH 7.4.
Endotoxin
Less than 1 EU/ug of rMuMIP-1β/CCL4 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
Purity
> 97 % by SDS-PAGE and HPLC analyses.
SDS-PAGE
Pack Size
10ug/100ug/500ug
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Murine Macrophage Inflammatory Protein-1 beta/CCL4 (rMuMIP-1beta/CCL4) on ChemWhat
