Recombinant Murine Macrophage Colony Stimulating Factor (rMuM-CSF)
Official Full Name
Recombinant Murine Macrophage Colony Stimulating Factor (rMuM-CSF)
Synonyms
CSF-1, MGI-IM
Squence
Amino Acid Sequence
KEVSEHCSHM IGNGHLKVLQ QLIDSQMETS CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN TPNANATERL QELSNNLNSC FTKDYEEQNK ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN NSFAKCSSRD VVTKPDCNCL YPKATPSSDP ASASPHQPPA PSMAPLAGLA WDDSQRTEGS SLLPSELPLR IEDPGSAKQR PPRSTCQTLE
Synonyms
CSF-1, MGI-IM
Accession
Gene ID
Summary
Source
Escherichia coli.
Molecular Weight
Approximately 52.0 kDa, a disulfide-linked homodimer consisting of two 230 amino acid polypeptide chains.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered solution in 20 mM Tris, 500 mM NaCl, pH 7.4.
Endotoxin
Less than 1 EU/ug of rMuM-CSF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
1. Cosman D, Wignall J, Anderson D, et al. 1988. Behring Inst Mitt: 15-26.2. Metcalf D, Willson T, Rossner M, et al. 1994. Growth Factors, 11: 145-52.3. Hidaka T, Fujimura M, Nakashima A, et al. 2002. Jpn J Cancer Res, 93: 426-35.4. Kubota Y, Takubo K, Shimizu T, et al. 2009. J Exp Med, 206: 1089-102.
Purity
> 95 % by SDS-PAGE and HPLC analyses.
SDS-PAGE
Pack Size
10ug/100ug/500ug
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Murine Macrophage Colony Stimulating Factor (rMuM-CSF) on ChemWhat
