Recombinant Murine Interleukin-22 (rMuIL-22)

Official Full Name

Recombinant Murine Interleukin-22 (rMuIL-22)

Synonyms

IL-TIF, IL-TIF alpha, IL-22a

Squence

Squence of Recombinant Murine Interleukin-22

Amino Acid Sequence

LPVNTRCKLE VSNFQQPYIV NRTFMLAKEA SLADNNTDVR LIGEKLFRGV SAKDQCYLMK QVLNFTLEDV LLPQSDRFQP YMQEVVPFLT KLSNQLSSCH ISGDDQNIQK NVRRLKETVK KLGESGEIKA IGELDLLFMS LRNACV

Synonyms

IL-TIF, IL-TIF alpha, IL-22a

Accession

Q9JJY9

Gene ID

50929

Summary

IL-22 is belonging to IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes and dendritic cells, IL-10 contributes to the inflammatory response in vivo. IL-22 signals through CRF2-4 and IL-22. It along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells. IL-22 likely plays a role in the coordinated response of both adaptive and innate immune systems. Recombinant mouse interleukin-22 contains 147 amino acids and it has approximately 81 % and 92 % amino acid sequence identity with human and rat IL-22.

Source

Escherichia coli.

Molecular Weight

Approximately 16.6 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by inducing IL-10 secretion of human COLO 205 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg.

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered concentrated solution in 1 × PBS, pH 7.4.

Endotoxin

Less than 1 EU/ug of rMuIL-22 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Purity

> 96 % by SDS-PAGE and HPLC analyses.

SDS-PAGE

SDS-PAGE of Recombinant Murine Interleukin-22

Pack Size

10ug/100ug/500ug

Safety Data Sheet (SDS) Download

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Technical Data Sheet (TDS) Download

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Links

This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Murine Interleukin-22 (rMuIL-22) on ChemWhat