Recombinant Murine Interleukin-1 Receptor Antagonist Protein (rMuIL-1RA)

Official Full Name

Recombinant Murine Interleukin-1 Receptor Antagonist Protein (rMuIL-1RA)

Synonyms

IL-1RN, IRAP

Squence

Squence of Recombinant Murine Interleukin-1 Receptor Antagonist Protein

Amino Acid Sequence

RPSGKRPCKM QAFRIWDTNQ KTFYLRNNQL IAGYLQGPNI KLEEKIDMVP IDLHSVFLGI HGGKLCLSCA KSGDDIKLQL EEVNITDLSK NKEEDKRFTF IRSEKGPTTS FESAACPGWF LCTTLEADRP VSLTNTPEEP LIVTKFYFQE DQ

Synonyms

IL-1RN, IRAP

Accession

Q542W1

Gene ID

16181

Summary

IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. IL-1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. The murine IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. The protein shows 26 % amino acid homology to IL-1β and 19% homology to IL-1alpha. It also shares 90 % a.a. sequence identity with rat IL-1RA.

Source

Escherichia coli.

Molecular Weight

Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg in the presence of 50 pg/ml rHuIL-1α.

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.

Endotoxin

Less than 1 EU/ug of rMuIL-1RA as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Purity

> 97 % by SDS-PAGE and HPLC analyses.

SDS-PAGE

SDS-PAGE of Recombinant Murine Interleukin-1 Receptor Antagonist Protein

Pack Size

100ug/500ug

Safety Data Sheet (SDS) Download

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Technical Data Sheet (TDS) Download

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Links

This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Murine Interleukin-1 Receptor Antagonist Protein (rMuIL-1RA) on ChemWhat