Recombinant Murine Fatty-acid-binding Protein 1 (rMuFABP1)
Official Full Name
Recombinant Murine Fatty-acid-binding Protein 1 (rMuFABP1)
Synonyms
14 kDa Selenium-binding Protein, Fatty Acid-binding Protein 1, L-FABP
Squence
Amino Acid Sequence
MNFSGKYQLQ SQENFEPFMK AIGLPEDLIQ KGKDIKGVSE IVHEGKKIKL TITYGPKVVR NEFTLGEECE LETMTGEKVK AVVKLEGDNK MVTTFKGIKS VTELNGDTIT NTMTLGDIVY KRVSKRI
Synonyms
14 kDa Selenium-binding Protein, Fatty Acid-binding Protein 1, L-FABP
Accession
Gene ID
Summary
Source
Escherichia coli.
Molecular Weight
Approximately 14.2 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids.
Biological Activity
Fully biologically active when compared to standard. The binding affinity of rMuFABP1 for the synthetic ligand cis-parinaric acid has been measured by fluorescence titration. Half maximal fluorescence of 2.5 uM rMuFABP1 is achieved with approximately 5 uM cis-paranaric acid.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 2 % trehalose.
Endotoxin
Less than 1 EU/ug of rMuFABP1 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
Purity
> 95 % by SDS-PAGE and HPLC analyses.
SDS-PAGE
Pack Size
5ug/100ug/500ug
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Murine Fatty-acid-binding Protein 1 (rMuFABP1) on ChemWhat
