Recombinant Human Novel Neurtrophin-1/B-Cell Stimulating Factor-3 (rHuNNT-1/BCSF-3)
Official Full Name
Recombinant Human Novel Neurtrophin-1/B-Cell Stimulating Factor-3 (rHuNNT-1/BCSF-3)
Synonyms
Cardiotrophin-like cytokine factor 1, BSF-3
Squence
Amino Acid Sequence
LNRTGDPGPG PSIQKTYDLT RYLEHQLRSL AGTYLNYLGP PFNEPDFNPP RLGAETLPRA TVDLEVWRSL NDKLRLTQNY EAYSHLLCYL RGLNRQAATA ELRRSLAHFC TSLQGLLGSI AGVMAALGYP LPQPLPGTEP TWTPGPAHSD FLQKMDDFWL LKELQTWLWR SAKDFNRLKK KMQPPAAAVT LHLGAHGF
Synonyms
Cardiotrophin-like cytokine factor 1, BSF-3
Accession
Gene ID
Summary
Source
Escherichia coli.
Molecular Weight
Approximately 22.3 kDa, a single non-glycosylated polypeptide chain containing 198 amino acids.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 4 ng/ml, corresponding to a specific activity of > 2.5 × 105 IU/mg in the presence of human CNTF R alpha.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Endotoxin
Less than 0.1 EU/ug of rHuNNT-1/BCSF-3 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
Purity
> 98 % by SDS-PAGE and HPLC analyses.
SDS-PAGE
Pack Size
10ug/100ug/500ug
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Human Novel Neurtrophin-1/B-Cell Stimulating Factor-3 (rHuNNT-1/BCSF-3) on ChemWhat
