Recombinant Human Monocyte Chemotactic Protein-4/CCL13 (rHuMCP-4/CCL13)

Official Full Name

Recombinant Human Monocyte Chemotactic Protein-4/CCL13 (rHuMCP-4/CCL13)

Synonyms

CK-beta-10, NCC-1, Small-inducible cytokine A13

Squence

Squence of Recombinant Human Monocyte Chemotactic Protein-4/CCL13

Amino Acid Sequence

QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT

Synonyms

CK-beta-10, NCC-1, Small-inducible cytokine A13

Accession

Q99616

Gene ID

6357

Summary

Source

Escherichia coli.

Molecular Weight

Approximately 8.6 kDa, a single non-glycosylated polypeptide chain containing 75 amino acids.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 10-100 ng/ml.

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.

Endotoxin

Less than 1 EU/ug of rHuMCP-4/CCL13 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

References

1. Uguccioni M, Loetscher P, Forssmann U, et al. 1996. J Exp Med. 183:2379-84.2. Berkhout TA, Sarau HM, Moores K, et al. 1997. J Biol Chem. 272:16404-13.3. Garcia-Zepeda EA, Combadiere C, Rothenberg ME, et al. 1996. J Immunol. 157:5613-26.

Purity

> 96 % by SDS-PAGE and HPLC analyses.

SDS-PAGE

SDS-PAGE of Recombinant Human Monocyte Chemotactic Protein-4/CCL13

Pack Size

5ug/100ug/500ug

Safety Data Sheet (SDS) Download

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Technical Data Sheet (TDS) Download

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Links

This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Human Monocyte Chemotactic Protein-4/CCL13 (rHuMCP-4/CCL13) on ChemWhat