Recombinant Human Macrophage Inflammatory Protein-3 alpha/CCL20 (rHuMIP-3alpha/CCL20)
Official Full Name
Recombinant Human Macrophage Inflammatory Protein-3 alpha/CCL20 (rHuMIP-3alpha/CCL20)
Synonyms
LARC, Beta-chemokine Exodus-1, Liver and Activation-regulated Chemokine
Squence
Amino Acid Sequence
ASNFDCCLGY TDRILHPKFI VGFTRQLANE GCDINAIIFH TKKKLSVCAN PKQTWVKYIV RLLSKKVKNM
Synonyms
LARC, Beta-chemokine Exodus-1, Liver and Activation-regulated Chemokine
Accession
Gene ID
Summary
CCL20, also named LARC (Liver and Activation-regulated Chemokine), is encoded by CCL20 located on chromosome 2 in humans. The expression can be induced by LPS and some inflammatory cytokines, and it is at a high level in lymph nodes, PBL, fatal liver, fatal lung, appendix and so on. CCL20 has functions of chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. CCL20 elicits it effects by binding with CCR6 receptor.
Source
Escherichia coli.
Molecular Weight
Approximately 8.0 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.
Biological Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-50 ng/ml.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
Endotoxin
Less than 1 EU/ug of rHuMIP-3α/CCL20 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
Purity
> 97 % by SDS-PAGE and HPLC analyses.
SDS-PAGE
Pack Size
5ug/100ug/500ug
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Human Macrophage Inflammatory Protein-3 alpha/CCL20 (rHuMIP-3alpha/CCL20) on ChemWhat
