Recombinant Human Macrophage Inflammatory Protein-1 beta/CCL4 (rHuMIP-1beta/CCL4)
Official Full Name
Recombinant Human Macrophage Inflammatory Protein-1 beta/CCL4 (rHuMIP-1beta/CCL4)
Synonyms
G-26 T-lymphocyte-secreted protein, HC21, LAG-1, MIP-1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible cytokine A4, ACT-2
Squence
Amino Acid Sequence
APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRSKQV CADPSESWVQ EYVYDLELN
Synonyms
G-26 T-lymphocyte-secreted protein, HC21, LAG-1, MIP-1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible cytokine A4, ACT-2
Accession
Gene ID
Summary
Source
Escherichia coli.
Molecular Weight
Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.
Biological Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 5.0-20 ng/ml.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Endotoxin
Less than 1 EU/ug of rHuMIP-1β/CCL4 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
1. Irving SG, Zipfel PF, Balke J, et al. 1990. Nucleic Acids Res. 18:3261-70.2. Cocchi F, DeVico AL, Garzino-Demo A, et al. 1995. Science. 270:1811-5.3. Garlisi CG, Xiao H, Tian F, et al. 1999. Eur J Immunol. 29:3210-5.4. Guan E, Wang J, Roderiquez G, et al. 2002. J Biol Chem. 277:32348-52.
Purity
> 96 % by SDS-PAGE and HPLC analyses.
SDS-PAGE
Pack Size
10ug/100ug/500ug
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Human Macrophage Inflammatory Protein-1 beta/CCL4 (rHuMIP-1beta/CCL4) on ChemWhat
