Recombinant Human Liver-Expressed Chemokine/CCL16 (rHuLEC/CCL16)

Official Full Name

Recombinant Human Liver-Expressed Chemokine/CCL16 (rHuLEC/CCL16)

Synonyms

HCC-4, LCC-1, LMC, MTN-1, NCC-4

Squence

Squence of Recombinant Human Liver-Expressed Chemokine/CCL16

Amino Acid Sequence

QPKVPEWVNT PSTCCLKYYE KVLPRRLVVG YRKALNCHLP AIIFVTKRNR EVCTNPNDDW VQEYIKDPNL PLLPTRNLST VKIITAKNGQ PQLLNSQ

Synonyms

HCC-4, LCC-1, LMC, MTN-1, NCC-4

Accession

O15467

Gene ID

6360

Summary

Source

Escherichia coli.

Molecular Weight

Approximately 11.2 kDa, a single non-glycosylated polypeptide chain containing 97 amino acids.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Endotoxin

Less than 1 EU/ug of rHuLEC/CCL16 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

References

1. Hedrick JA, Helms A, Vicari A, et al. 1998. Blood, 91: 4242-7.2. Nomiyama H, Fukuda S, Iio M, et al. 1999. J Interferon Cytokine Res, 19: 227-34.3. Youn BS, Zhang S, Broxmeyer HE, et al. 1998. Biochem Biophys Res Commun, 247: 217-22.4. Kim IS, Jang SW, Sung HJ, et al. 2005. FEBS Lett, 579: 6044-8.5. Francica G, Petrolati A, Di Stasio E, et al. 2012. Acta Radiol, 53: 394-400.

Purity

> 97 % by SDS-PAGE and HPLC analyses.

SDS-PAGE

SDS-PAGE of Recombinant Human Liver-Expressed Chemokine/CCL16

Pack Size

5ug/100ug/500ug

Safety Data Sheet (SDS) Download

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Technical Data Sheet (TDS) Download

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Links

This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Human Liver-Expressed Chemokine/CCL16 (rHuLEC/CCL16) on ChemWhat