Recombinant Human Keratinocyte Growth Factor-2/FGF-10 (rHuKGF-2/FGF-10)

Official Full Name

Recombinant Human Keratinocyte Growth Factor-2/FGF-10 (rHuKGF-2/FGF-10)

Synonyms

Squence

Squence of Recombinant Human Keratinocyte Growth Factor-2/FGF-10

Amino Acid Sequence

LGQDMVSPEA TNSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS

Synonyms

Accession

O15520

Gene ID

2255

Summary

Source

Molecular Weight

Approximately 19.1 kDa, a single, non-glycosylated polypeptide chain containing 169 amino acids.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered concentrated solution in 2 × PBS, pH 7.4.

Endotoxin

Less than 1 EU/ug of rHuKGF-2/FGF-10 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

References

1. Emoto H, Tagashira S, Mattei MG, et al. 1997. J Biol Chem. 272:23191-4.2. Tagashira S, Harada H, Katsumata T, et al. 1997. Gene. 197:399-404.3. Carninci P, Kasukawa T, Katayama S, et al. 2005. Science. 309:1559-63.4. Igarashi M, Finch PW, Aaronson SA. 1998. J Biol Chem. 273:13230-5.5. Entesarian M, Matsson H, Klar J, et al. 2005. Nat Genet. 37:125-7.

Purity

> 97 % by SDS-PAGE and HPLC analyses.

SDS-PAGE

SDS-PAGE of Recombinant Human Keratinocyte Growth Factor-2/FGF-10

Pack Size

5ug/100ug/500ug

Safety Data Sheet (SDS) Download

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Technical Data Sheet (TDS) Download

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Links

This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Human Keratinocyte Growth Factor-2/FGF-10 (rHuKGF-2/FGF-10) on ChemWhat