Recombinant Human Interleukin-15 GMP (rHuIL-15)
Official Full Name
Recombinant Human Interleukin-15 GMP (rHuIL-15)
Synonyms
IL-T
Squence
Amino Acid Sequence
NWVNVISDLK KIEDLIQSMH IDATLYTESD VHPSCKVTAM KCFLLELQVI SLESGDASIH DTVENLIILA NNSLSSNGNV TESGCKECEE LEEKNIKEFL QSFVHIVQMF INTS
Synonyms
IL-T
Accession
Gene ID
Summary
Human Interleukin-15 (IL-15) is expressed by the IL15 gene located on the chromosome 4. It shares approximately 97 % and 73 % sequence identity with simian and murine IL-15, respectively. Both human and simian IL-15 are active on murine cells. IL-15 is secreted by mononuclear phagocytes (and some other cells), especially macrophages following infection by virus. It possesses a variety of biological functions, including stimulating and maintaining of cellular immune responses, especially regulating T and natural killer (NK) cell activation and proliferation. In additionally, it shares many biological properties with IL-2, including T, B and NK cell-stimulatory activities. IL-15 signals through a complex composed of IL-2/IL-15 receptor beta chain. Although IL-15 lacks sequence homology with IL-2, it has recently been shown that both the beta and gamma chains of the IL-2 receptor are utilized for IL-15 binding and signaling. In addition, an IL-15 specific binding protein has also been cloned from a mouse T cell clone.
Source
Escherichia coli.
Molecular Weight
Approximately 12.9 kDa, a single non-glycosylated polypeptide chain containing 114 amino acids.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Endotoxin
Less than 0.01 EU/ug of rHuIL-15 GMP as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
1. Anderson DM, Johnson L, Glaccum MB, et al. 1995. Genomics, 25: 701-6.2. Krause H, Jandrig B, Wernicke C, et al. 1996. Cytokine, 8: 667-74.3. Chirifu M, Hayashi C, Nakamura T, et al. 2007. Nat Immunol, 8: 1001-7.4. Grabstein KH, Eisenman J, Shanebeck K, et al. 1994. Science, 264: 965-8.5. Giri JG, Ahdieh M, Eisenman J, et al. 1994. EMBO J, 13: 2822-30.6. Arena A, Merendino RA, Bonina L, et al. 2000. New Microbiol, 23: 105-12.
Purity
> 98 % by SDS-PAGE and HPLC analyses.
SDS-PAGE
Pack Size
5ug/100ug
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Human Interleukin-15 GMP (rHuIL-15) on ChemWhat
