Recombinant Human Interferon-alpha2b, Ecoli. (rHuIFN-alpha2b,Ecoli.)

Official Full Name

Recombinant Human Interferon-alpha2b, Ecoli. (rHuIFN-alpha2b,Ecoli.)

Synonyms

Squence

Squence of Recombinant Human Interferon-alpha2b, Ecoli.

Amino Acid Sequence

MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE

Synonyms

Accession

P01563

Gene ID

3440

Summary

IFN-alphas are proteins secreted by leukocyte. They are mainly involved in innate immune response against viral infection. The IFN-alpha family has 13 subtypes and 23 different variants. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.

Source

Escherichia coli.

Molecular Weight

Approximately 19.4 kDa, a single non-glycosylated polypeptide chain containing 166 amino acids.

Biological Activity

Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.0 × 108 IU/mg.

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.

Endotoxin

Less than 1 EU/ug of rHuIFN-α2b, Ecoli. as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Purity

> 96 % by SDS-PAGE and HPLC analyses.

SDS-PAGE

SDS-PAGE of Recombinant Human Interferon-alpha2b, Ecoli.

Pack Size

100ug/500ug

Safety Data Sheet (SDS) Download

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Technical Data Sheet (TDS) Download

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Links

This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Human Interferon-alpha2b, Ecoli. (rHuIFN-alpha2b,Ecoli.) on ChemWhat