Recombinant Human Indian Hedgehog Cys28IleIle (rHuIHHC28II)
Official Full Name
Recombinant Human Indian Hedgehog Cys28IleIle (rHuIHHC28II)
Synonyms
HHG-2
Squence
Amino Acid Sequence
IIGPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG
Synonyms
HHG-2
Accession
Gene ID
Summary
Source
Escherichia coli.
Molecular Weight
Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10 ug/ml.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in 1 × PBS, pH 7.4.
Endotoxin
Less than 1 EU/ug of rHuIHH C28II as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
1. Kumar S, Balczarek KA, Lai ZC. 1996. Genetics. 142:965-72.2. Marigo V, Roberts DJ, Lee SM, et al. 1995. Genomics. 28:44-51.3. Vortkamp A, Lee K, Lanske B, et al. 1996. Science. 273:613-22.
Purity
> 96 % by SDS-PAGE and HPLC analyses.
SDS-PAGE
Pack Size
5ug/100ug/500ug
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Human Indian Hedgehog Cys28IleIle (rHuIHHC28II) on ChemWhat
