Recombinant Human Heparin-binding EGF-like Growth Factor (rHuHB-EGF)

Official Full Name

Recombinant Human Heparin-binding EGF-like Growth Factor (rHuHB-EGF)

Synonyms

HBEGF, DT-R

Squence

Squence of Recombinant Human Heparin-binding EGF-like Growth Factor

Amino Acid Sequence

DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL

Synonyms

HBEGF, DT-R

Accession

Q99075

Gene ID

1839

Summary

Heparin-binding epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation, wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HB-EGF is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. Human HB-EGF shares about 73 % and 76 % a.a. sequence identity with murine and rat HB-EGF.

Source

Escherichia coli.

Molecular Weight

Approximately 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg.

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.

Endotoxin

Less than 1 EU/ug of rHuHB-EGF as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Purity

> 97 % by SDS-PAGE and HPLC analyses.

SDS-PAGE

SDS-PAGE of Recombinant Human Heparin-binding EGF-like Growth Factor

Pack Size

10ug/100ug/500ug

Safety Data Sheet (SDS) Download

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Technical Data Sheet (TDS) Download

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Links

This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Human Heparin-binding EGF-like Growth Factor (rHuHB-EGF) on ChemWhat