Recombinant Human Hemofiltrate CC Chemokine-1/CCL14 (rHuHCC-1/CCL14)
Official Full Name
Recombinant Human Hemofiltrate CC Chemokine-1/CCL14 (rHuHCC-1/CCL14)
Synonyms
HCC-1/HCC-3, NCC-2, Small-inducible Cytokine A14
Squence
Amino Acid Sequence
TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN
Synonyms
HCC-1/HCC-3, NCC-2, Small-inducible Cytokine A14
Accession
Gene ID
Summary
Source
Escherichia coli.
Molecular Weight
Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 72 amino acids.
Biological Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.0-20 ng/ml.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
Endotoxin
Less than 1 EU/ug of rHuHCC-1/CCL14 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
1. Schulz-Knappe P, Magert HJ, Dewald B, et al. 1996. J Exp Med. 183:295-9.2. Detheux M, Standker L, Vakili J, et al. 2000. J Exp Med. 192:1501-8.3. Pardigol A, Forssmann U, Zucht HD, et al. 1998. Proc Natl Acad Sci U S A. 95:6308-13.
Purity
> 96 % by SDS-PAGE and HPLC analyses.
SDS-PAGE
Pack Size
10ug/100ug/500ug
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Human Hemofiltrate CC Chemokine-1/CCL14 (rHuHCC-1/CCL14) on ChemWhat
