Recombinant Human Hemofiltrate CC Chemokine-1, 66a.a./CCL14 (rHuHCC-1,66a.a./CCL14)

Official Full Name

Recombinant Human Hemofiltrate CC Chemokine-1, 66a.a./CCL14 (rHuHCC-1,66a.a./CCL14)

Synonyms

C-C Motif Chemokine 14, HCC-3, NCC-2, Small-inducible Cytokine A14

Squence

Squence of Recombinant Human Hemofiltrate CC Chemokine-1, 66a.a./CCL14

Amino Acid Sequence

GPYHPSECCF TYTTYKIPRQ RIMDYYETNS QCSKPGIVFI TKRGHSVCTN PSDKWVQDYI KDMKEN

Synonyms

C-C Motif Chemokine 14, HCC-3, NCC-2, Small-inducible Cytokine A14

Accession

Q16627

Gene ID

6358

Summary

Human CCL14 is belonging to the CC chemokine family. It is encoded by the gene CCL14. CCL14 has two isoforms, CCL14a (HCC-1) and CCL14b (HCC-3). The sequence of HCC-3 differs from HCC-1 as follow: 27-27 R→ QTGGKPKVVKIQLKLVG. CCL14 was first isolated from the hemofiltrate of human patients with chronic renal failure. The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood. CCL14 promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1 and is a ligand for CCR1, CCR3 and CCR5. Recombinant human CCL14 (66 a.a.) contains 66 amino acid residues and activation of the HCC 1/CCL14a precursor to active peptide is mediated by the urokinase type plasminogen activator or plasmin.

Source

Escherichia coli.

Molecular Weight

Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 66 amino acids.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 5.0-20 ng/ml.

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered concentrated solution in 2 × PBS, pH 7.4, 5 % trehalose.

Endotoxin

Less than 1 EU/ug of rHuHCC-1, 66a.a./CCL14 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Purity

> 95 % by SDS-PAGE and HPLC analyses.

SDS-PAGE

SDS-PAGE of Recombinant Human Hemofiltrate CC Chemokine-1, 66a.a./CCL14

Pack Size

10ug/100ug/500ug

Safety Data Sheet (SDS) Download

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Technical Data Sheet (TDS) Download

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Links

This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Human Hemofiltrate CC Chemokine-1, 66a.a./CCL14 (rHuHCC-1,66a.a./CCL14) on ChemWhat