Recombinant Human Fibroblast Growth Factor 4 (rHuFGF-4)

Official Full Name

Recombinant Human Fibroblast Growth Factor 4 (rHuFGF-4)

Synonyms

HST, HST-1, HSTF-1, HBGF-4, Transforming Protein KS3

Squence

Squence of Recombinant Human Fibroblast Growth Factor 4

Amino Acid Sequence

GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL

Synonyms

HST, HST-1, HSTF-1, HBGF-4, Transforming Protein KS3

Accession

P08620

Gene ID

2249

Summary

FGF-4, also named FGF­K and K­FGF, belongs to the fibroblast growth factor (FGF) family. By signaling through the FGF R1c, 2c, 3c and 4 receptors, FGF-4 has functions that maintain a population of progenitor cells in the epiblast that generates mesoderm, and contribute to the stem cell population that is incorporated in the tailbud. It is also required for axial elongation of the mouse embryo after gastrulation. Mature human FGF­4 (71­206 a.a.) shares 91 %, 82 %, 94 % and 91 % a.a. identity with murine, rat, canine and bovine FGF­4. Additionally, FGF-4 shares about 30 % sequence identity with the prototypical members of the FGF family.

Source

Escherichia coli.

Molecular Weight

Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered concentrated solution in 1 × PBS, pH 7.4, 300 mM NaCl.

Endotoxin

Less than 1 EU/ug of rHuFGF-4 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 500 mM NaCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Purity

> 96 % by SDS-PAGE and HPLC analyses.

SDS-PAGE

SDS-PAGE of Recombinant Human Fibroblast Growth Factor 4

Pack Size

5ug/100ug/500ug

Safety Data Sheet (SDS) Download

Click to download

Technical Data Sheet (TDS) Download

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Links

This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Human Fibroblast Growth Factor 4 (rHuFGF-4) on ChemWhat