Official Full Name
Recombinant Human Ciliary Neurotrophic Factor (rHuCNTF)
Amino Acid Sequence
MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM
Approximately 22.9 kDa, a single non-glycosylated polypeptide chain containing 200 amino acids.
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 × 103 IU/mg.
Sterile filtered white lyophilized (freeze-dried) powder.
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Less than 1 EU/ug of rHuCNTF as determined by LAL method.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
1. Lam A, Fuller F, Miller J, et al. 1991. Gene. 102:271-6.2. Bazan JF. 1991. Neuron. 7:197-208.3. McGregor NE, Poulton IJ, Walker EC, et al. 2010. Calcif Tissue Int. 86:261-70.4. Lambert PD, Anderson KD, Sleeman MW, et al. 2001. Proc Natl Acad Sci U S A. 98:4652-7.
> 97 % by SDS-PAGE and HPLC analyses.
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
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