Official Full Name
Recombinant Human Beta-defensin 3 (rHuBD-3)
Amino Acid Sequence
GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK
Approximately 5.2 kDa, a single non-glycosylated polypeptide chain containing 45 amino acids.
Fully biologically active when compared to standard. The ED50 as determined by anti-microbial activity against E.coli. is less than 30 ug/ml, corresponding to a specific activity of > 33.3 IU/mg.
Sterile filtered white lyophilized (freeze-dried) powder.
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.
Less than 1 EU/ug of rHuBD-3 as determined by LAL method.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
1. Lioi AB, Reyes Rodriguez AL, Funderburg NT, et al. 2012. J Leukoc Biol, 92: 1083-91.2. Wang K, Wang JH, Baskaran H, et al. 2012. Head Neck Oncol, 4: 41.3. Lee JK, Park YJ, Kum KY, et al. 2012. Int Endod J,
> 98 % by SDS-PAGE and HPLC analyses.
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
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