Recombinant Human Beta-defensin 3 (rHuBD-3)
Official Full Name
Recombinant Human Beta-defensin 3 (rHuBD-3)
Synonyms
Defensin beta3
Squence
Amino Acid Sequence
GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK
Synonyms
Defensin beta3
Accession
Gene ID
Summary
Source
Escherichia coli.
Molecular Weight
Approximately 5.2 kDa, a single non-glycosylated polypeptide chain containing 45 amino acids.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by anti-microbial activity against E.coli. is less than 30 ug/ml, corresponding to a specific activity of > 33.3 IU/mg.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.
Endotoxin
Less than 1 EU/ug of rHuBD-3 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
1. Lioi AB, Reyes Rodriguez AL, Funderburg NT, et al. 2012. J Leukoc Biol, 92: 1083-91.2. Wang K, Wang JH, Baskaran H, et al. 2012. Head Neck Oncol, 4: 41.3. Lee JK, Park YJ, Kum KY, et al. 2012. Int Endod J,
Purity
> 98 % by SDS-PAGE and HPLC analyses.
SDS-PAGE
Pack Size
5ug/100ug/500ug
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Human Beta-defensin 3 (rHuBD-3) on ChemWhat
