Recombinant Human B-Cell Maturation Antigen/TNFRSF17 (rHuBCMA/TNFRSF17)
Official Full Name
Recombinant Human B-Cell Maturation Antigen/TNFRSF17 (rHuBCMA/TNFRSF17)
Synonyms
TNFRSF17, BCM, CD269
Squence
Amino Acid Sequence
AGQCSQNEYF DSLLHACIPC QLRCSSNTPP LTCQRYCNAS VTNSVKGTNA
Synonyms
TNFRSF17, BCM, CD269
Accession
Gene ID
Summary
Source
Escherichia coli.
Molecular Weight
Approximately 5.4 kDa, a single non-glycosylated polypeptide chain containing 50 amino acids.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit APRIL-mediated proliferation of anti-IgM stimulated murine B cells is no less than 40 ng/ml, corresponding to a specific activity of > 2.5 × 104 IU/mg in the presence of 100 ng/ml human APRIL.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA.
Endotoxin
Less than 1 EU/ug of rHuBCMA as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
1. Kim J, Gross JA, Dillon SR, et al. 2011. Autoimmunity, 44: 69-81.2. Sun J, Feng J, Li Y, et al. 2006. Biochem Biophys Res Commun, 346: 1158-62.3. Pelletier M, Thompson JS, Qian F, et al. 2003. J Biol Chem, 278: 33127-33.4. Laabi Y, Gras MP, Brouet JC, et al. 1994. Nucleic Acids Res, 22: 1147-54.
Purity
> 98 % by SDS-PAGE and HPLC analyses.
SDS-PAGE
Pack Size
5ug/100ug/500ug
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Human B-Cell Maturation Antigen/TNFRSF17 (rHuBCMA/TNFRSF17) on ChemWhat
