Recombinant Hirudin (rHirudin)

Official Full Name

Recombinant Hirudin (rHirudin)

Synonyms

Squence

Squence of Recombinant Hirudin

Amino Acid Sequence

VVYTDCTESG QNLCLCEGSN VCGQGNKCIL GSDGEKNQCV TGEGTPGPQS HNDGDFEEPE EYL

Synonyms

Accession

P84590

Gene ID

Summary

Source

Pichia Pastoris

Molecular Weight

Approximately 6.7 kDa, a single non-glycosylated polypeptide chain containing 63 amino acid residues.

Biological Activity

The biological activity is determined by chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific activity is no less than 14,000 ATU/mg protein.

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered solution of 20 mM PBS, pH 7.0, containing 2 % mannitol.

Endotoxin

Less than 1 EU/ug of rHirudin as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

References

1. Knapp A, Degenhardt T, Dodt J. 1992. J Biol Chem, 267: 24230-4.2. Pineo GFandHull RD. 1995. Curr Opin Hematol, 2: 380-5.3. Badimon L, Merino A, Badimon J, et al. 1991. Trends Cardiovasc Med, 1: 261-7.4. Nasir MA, Toth CA, Mittra RA. 1996. Am J Ophthalmol, 121: 554-60.5. Agnelli GandSonaglia F. 1997. Semin Thromb Hemost, 23: 143-8.

Purity

> 96 % by SDS-PAGE and HPLC analyses.

SDS-PAGE

SDS-PAGE of Recombinant Hirudin

Pack Size

10ug/100ug/500ug

Safety Data Sheet (SDS) Download

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Technical Data Sheet (TDS) Download

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Links

This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Hirudin (rHirudin) on ChemWhat