Official Full Name
Recombinant Hirudin (rHirudin)
Amino Acid Sequence
VVYTDCTESG QNLCLCEGSN VCGQGNKCIL GSDGEKNQCV TGEGTPGPQS HNDGDFEEPE EYL
Approximately 6.7 kDa, a single non-glycosylated polypeptide chain containing 63 amino acid residues.
The biological activity is determined by chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific activity is no less than 14,000 ATU/mg protein.
Sterile filtered white lyophilized (freeze-dried) powder.
Lyophilized from a 0.2 um filtered solution of 20 mM PBS, pH 7.0, containing 2 % mannitol.
Less than 1 EU/ug of rHirudin as determined by LAL method.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
1. Knapp A, Degenhardt T, Dodt J. 1992. J Biol Chem, 267: 24230-4.2. Pineo GFandHull RD. 1995. Curr Opin Hematol, 2: 380-5.3. Badimon L, Merino A, Badimon J, et al. 1991. Trends Cardiovasc Med, 1: 261-7.4. Nasir MA, Toth CA, Mittra RA. 1996. Am J Ophthalmol, 121: 554-60.5. Agnelli GandSonaglia F. 1997. Semin Thromb Hemost, 23: 143-8.
> 96 % by SDS-PAGE and HPLC analyses.
Safety Data Sheet (SDS) Download
Technical Data Sheet (TDS) Download
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