Recombinant Carboxypeptidase B EC 3.4.17.2 CAS 9025-24-5
Identification
CAS Number
9025-24-5
EC Number
3.4.17.2
Name
Recombinant Carboxypeptidase B
Synonyms
N-(3-Carboxypropanoyl)alanylalanyl-N-[1-(benzylsulfanyl)-1-oxo-3-phenyl-2-propanyl]prolinamid [German] [ACD/IUPAC Name]
N-(3-Carboxypropanoyl)alanylalanyl-N-[1-(benzylsulfanyl)-1-oxo-3-phenyl-2-propanyl]prolinamide [ACD/IUPAC Name]
N-(3-Carboxypropanoyl)alanylalanyl-N-[1-(benzylsulfanyl)-1-oxo-3-phényl-2-propanyl]prolinamide [French] [ACD/IUPAC Name]
Prolinamide, N-(3-carboxy-1-oxopropyl)alanylalanyl-N-[2-oxo-1-(phenylmethyl)-2-[(phenylmethyl)thio]ethyl]- [ACD/Index Name]
3-[(1-{[1-(2-{[1-(BENZYLSULFANYL)-1-OXO-3-PHENYLPROPAN-2-YL]CARBAMOYL}PYRROLIDIN-1-YL)-1-OXOPROPAN-2-YL]CARBAMOYL}ETHYL)CARBAMOYL]PROPANOIC ACID
4-[[1-[[1-[2-[(1-benzylsulfanyl-1-oxo-3-phenylpropan-2-yl)carbamoyl]pyrrolidin-1-yl]-1-oxopropan-2-yl]amino]-1-oxopropan-2-yl]amino]-4-oxobutanoic acid
9025-24-5 [RN]
Carboxypeptidase B from human pancreas
Carboxypeptidase- B porcine pancreas
Protaminase
SMILES
CC(C(=O)NC(C)C(=O)N1CCCC1C(=O)NC(Cc2ccccc2)C(=O)SCc3ccccc3)NC(=O)CCC(=O)O
InChi
InChI=1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
InChIKey
TWURVFFNODFJBJ-UHFFFAOYSA-N
Molecular Formula
C31H38N4O7S
Molecular Weight
610.721
EINECS
232-788-9
MDL Number
MFCD00081476
Properties
Appearance
White or off white, or yellowish powder
Safety Data
Symbol
GHS07,GHS08Signal Word
Danger
Hazard statements
H315 – H319 – H334 – H335Precautionary Statements
P302 + P352 – P305 + P351 + P338Target organs
Respiratory system
Personal Protective Equipment
Eyeshields, Gloves
RIDADR
NONH for all modes of transport
WGK Germany
3
Specifications and Other Information of Our Recombinant Carboxypeptidase B EC 3.4.17.2 CAS 9025-24-5
| Items | Recombinant Carboxypeptidase B | Sequencing Grade Carboxypeptidase B |
| Specific activity | ≥170 units/mg pro. | ≥200 units/mg pro. |
| SDS-PAGE | Single major band | Single major band |
| Molecular Weight(SDS-PAGE) | 33.8±3.4 kDa | 33.8±3.4 kDa |
| Purity(HPLC) | / | ≥ 95% |
| Unit definition | One unit of carboxypeptidase B activity hydrolyzes one micromole ofhippuryl-L-arginine per minute at 25℃, pH 7.65. | Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0. |
| Recommended usage | Prepare 1-10mg/ml recombinant carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0. | / |
Description
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA.
Source
It is a genetically engineered protein expressed in E.coli
Protein Sequence
The amino acid sequence of recombinant carboxypeptidase B is identical to rat carboxypeptidase B
Stability of storage
Sequencing grade carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃. Avoid repeated freezing and thawing.
Stability of transport
The product is stable by blue ice insulation transport.
Advantage
- Animal origin free: recombinant trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
- Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
- High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
- Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
Known Application
The production of recombinant insulin and its analogues; the quality test of recombinant antibodies; the determination of C-terminal amino acids of proteins; the production of other recombinant polypeptides; and the enzymatic synthesis of certain specific compounds. Determination of protein C-terminal basic amino acids such as arginine, lysine and histidine; detection of antibody basic peak.
