Recombinant Carboxypeptidase B
N-(3-Carboxypropanoyl)alanylalanyl-N-[1-(benzylsulfanyl)-1-oxo-3-phenyl-2-propanyl]prolinamid [German] [ACD/IUPAC Name]
N-(3-Carboxypropanoyl)alanylalanyl-N-[1-(benzylsulfanyl)-1-oxo-3-phenyl-2-propanyl]prolinamide [ACD/IUPAC Name]
N-(3-Carboxypropanoyl)alanylalanyl-N-[1-(benzylsulfanyl)-1-oxo-3-phényl-2-propanyl]prolinamide [French] [ACD/IUPAC Name]
Prolinamide, N-(3-carboxy-1-oxopropyl)alanylalanyl-N-[2-oxo-1-(phenylmethyl)-2-[(phenylmethyl)thio]ethyl]- [ACD/Index Name]
Carboxypeptidase B from human pancreas
Carboxypeptidase- B porcine pancreas
White or off white, or yellowish powder
Hazard statementsH315 – H319 – H334 – H335
Precautionary StatementsP302 + P352 – P305 + P351 + P338
Personal Protective Equipment
NONH for all modes of transport
Specifications and Other Information of Our Recombinant Carboxypeptidase B EC 188.8.131.52 CAS 9025-24-5
|Items||Recombinant Carboxypeptidase B||Sequencing Grade Carboxypeptidase B|
|Specific activity||≥170 units/mg pro.||≥200 units/mg pro.|
|SDS-PAGE||Single major band||Single major band|
|Molecular Weight(SDS-PAGE)||33.8±3.4 kDa||33.8±3.4 kDa|
|Unit definition||One unit of carboxypeptidase B activity hydrolyzes one micromole ofhippuryl-L-arginine per minute at 25℃, pH 7.65.||Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0.|
|Recommended usage||Prepare 1-10mg/ml recombinant carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0.||/|
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA.
It is a genetically engineered protein expressed in E.coli
The amino acid sequence of recombinant carboxypeptidase B is identical to rat carboxypeptidase B
Stability of storage
Sequencing grade carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃. Avoid repeated freezing and thawing.
Stability of transport
The product is stable by blue ice insulation transport.
- Animal origin free: recombinant trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
- Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
- High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
- Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
The production of recombinant insulin and its analogues; the quality test of recombinant antibodies; the determination of C-terminal amino acids of proteins; the production of other recombinant polypeptides; and the enzymatic synthesis of certain specific compounds. Determination of protein C-terminal basic amino acids such as arginine, lysine and histidine; detection of antibody basic peak.