Poly(A) Polymerase CAS 9026-30-6
Identification
CAS Number
9026-30-6
EC Number
2.7.7.19
Name
Poly(A) Polymerase
Synonyms
Polynucleotide Adenylyltransferase
ATP:polynucleotide adenylyltransferase
ATP RNA Adenylyltransferase
ATP-RNA Adenylyltransferase
Adenylyltransferase, ATP-RNA
Adenylyltransferase, polynucleotide
Poly A Polymerase
Polyadenylate polymerase
Polyadenylate synthetase
MDL Number
MFCD00166322
Properties
Optimal reaction temperature
37℃
Definition of active unit
Definition of active unit: 1 active unit is defined as the amount of enzyme needed to incorporate 1 nmol of AMP into RNA within 10min at 37℃ in a 20μL reaction system.
Safety Data
RIDADR
NONH for all modes of transport
WGK Germany
3
Specifications and Other Information of Our Poly(A) Polymerase CAS 9026-30-6
Product Introduction
This product is the Poly(A) Polymerase recombinantly expressed in E. coli. It catalyzes with ATP as the substrate, and adds adenyl acid to the 3’-hydroxyl terminus of RNA to form the PolyA tail structure in the form of AMP.
Product Features
Efficient mRNA capping: adding 200nt polyA tail to 10μg of RNA in 10min (5U enzyme for capping). Homogenous tailing length: controlling polyA tail length by adjusting reaction time or enzyme dosage.
Product Composition
| Item No. | Composition | Storage Temperature (℃ ) | 100U | 1000U |
| I | Poly(A) Polymerase (50U/μL) | -20 | 40μL | 400μL |
| Ⅱ | Reaction Buffer | -20 | 1.5mL | 15mL |
| Ⅲ | AdENosine-5’-Triphosphate (ATP10mM) | -20 | 200μL | 2000μL |
1x Reaction Buffer contains 0.5M Tris-HCl(pH8.0), 50mM KCl and 10mM MgCl2.
Quality Control
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
Links
This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).