mRNA Cap-2′-O-Methyltransferase CAS 61970-02-3
Identification
CAS Number
61970-02-3
EC Number
2.1.1.57
Name
mRNA Cap-2′-O-Methyltransferase
Properties
Optimal reaction temperature
37℃
Definition of active unit
Definition of active unit: 1 active unit is defined as the amount of enzyme needed to incorporate 10 pmol of (α32P)GTP into 80nt transcript within 1h at 37℃ .
Safety Data
RIDADR
NONH for all modes of transport
WGK Germany
3
Specifications and Other Information of Our mRNA Cap-2′-O-Methyltransferase CAS 61970-02-3
Product Introduction
This product is the mRNA Cap-2′-O-Methyltransferase of vaccinia virus recombinantly expressed in E. coli. With the RNA of 7-methylguanosine cap (Cap0) as the substrate, it specifically transfers the methyl of methylation donator SAM to the Cap0 structure in RNA to form the Cap1 structure, which can enhance the translation efficiency of mRNA and avoid the innate immune response in certain cells.
Product Features
In vitro capping can avoid in vivo immune response.
Product Composition
| Item No. | Composition | Storage Temperature (℃ ) | 2KU | 20KU |
| I | mRNA Cap-2′-O-Methyltransferase(50U/μL) | -20 | 40μL | 400μL |
| Ⅱ | Capping Buffer | -20 | 100μL | 1000μL |
| Ⅲ | S-adENosylmethionine (SAM 32mM) | -20 | 100μL | 1000μL |
10× Capping Buffer contains: 0.5M Tris-HCl(pH8.0), 50mM KCl, 10mM MgCl2 and 10mM DTT.
Quality Control
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
Links
This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).