mRNA Cap-2′-O-Methyltransferase CAS 61970-02-3
Optimal reaction temperature
Definition of active unit
Definition of active unit: 1 active unit is defined as the amount of enzyme needed to incorporate 10 pmol of (α32P)GTP into 80nt transcript within 1h at 37℃ .
NONH for all modes of transport
Specifications and Other Information of Our mRNA Cap-2′-O-Methyltransferase CAS 61970-02-3
This product is the mRNA Cap-2′-O-Methyltransferase of vaccinia virus recombinantly expressed in E. coli. With the RNA of 7-methylguanosine cap (Cap0) as the substrate, it specifically transfers the methyl of methylation donator SAM to the Cap0 structure in RNA to form the Cap1 structure, which can enhance the translation efficiency of mRNA and avoid the innate immune response in certain cells.
In vitro capping can avoid in vivo immune response.
|Item No.||Composition||Storage Temperature|
|Ⅲ||S-adENosylmethionine (SAM 32mM)||-20||100μL||1000μL|
10× Capping Buffer contains: 0.5M Tris-HCl(pH8.0), 50mM KCl, 10mM MgCl2 and 10mM DTT.
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).