L-Rhamnosidase CAS 37288-35-0


CAS Number


EC Number




Native Penicillium sp. α-Rhamnosidase;
α-Rhamnosidase 78A from Streptomyces avermitilis, Recombinant;
Native Thermomicrobia sp. Naringinase (Rhamnosidase A);



White powder

Safety Data


NONH for all modes of transport

WGK Germany


Specifications and Other Information of Our L-Rhamnosidase CAS 37288-35-0


The product is prepared by submerged fermentation of Aspergillus niger. a-L-rhamnosidase (EC3.2.1.40) is a specific glycoside hydrolase that acts on a-1,2, a-1,3, a-1,4, a-1,6 and a 1-linked glycosidic bonds. and a 1-linked glycosidic bonds, hydrolysing the non-reducing a-L-rhamnosidic bond bound to the end of the substrate, while releasing rhamnose and the corresponding ligand. a-L-rhamnosidase acts synergistically with β-glucosidase to specifically hydrolyse some natural products such as naringin, rutin and quercetin to remove bitterness.

Source of strains

Aspergillus niger

Rhamnosidase activity U/g≥50000
Sensory indicatorsLight yellow powder ( colour may vary slightly from batch to batch due to fermentation raw materials, cycles etc. but does not affect the efficacy of use)
Weight loss on drying(%)≤8.0
Fineness (80 mesh standard sieve pass rate) (%)≥90
Total arsenic (as As)/(mg/kg)≤3
Total number of bacterial colonies/(CFU/g)≤50000
Coliform bacteria(CFU/g)≤30
Escherichia coli (MPN/g)≤3.0
Salmonella (25g)Not detected

Product Efficacy

1.Plant extracts can replace acid and alkaline hydrolysis, reducing environmental pollution and increasing the yield of active ingredients;
2.It is used to remove the bitterness of naringin from citrus juice and to hydrolyse naringin into rhamnose and tangerine glycosides, of which tangerine glycosides are important precursors for the industrial production of sweeteners.

3.Acts on terpene glycosides to improve the aroma composition of grape juice, wine and beverages, etc. and to produce food additives;
4.It can hydrolyse naringin and lemon bitterness in fruits of the Rutaceae family to remove bitterness from the juice, thus making the juice sweet and sour with a pleasant taste;
5.It can improve the bioavailability of flavonoids and can be used in the production of functional drinks;
6.Catalytic hydrolysis of rutinoside for the targeted biosynthesis of isoquercitrin, which could be a hot new drug compound;
7.Catalyzes the synthesis of naringin, which can be used as a raw material for a variety of pharmaceuticals.

Enzvmatic Properties

Temperature of action: optimum 50℃-60℃, effective 30℃-90℃.
pH of action: optimum pH 4.0-5.0, effective pH 3.0-8.0.


Safety: natural fermented sources for a high level of safety.
Highly effective: soluble in water, highly active, specific and effective.

Directions For Use And Recommended Dosage

Add directly or to the reaction solution.
The recommended dosage is 0.01 %-0.5%, the optimum dosage is determined after testing according to the process characteristics.


1 kg/pack, Skg/pack, 25kg/pack


Store in a cool, dry environment, out of direct sunlight; keep intact or in closed containers when storing.

Shelf Life

24 months

This product is developed by our R&D company Watson International Ltd (https://www.watson-int.com/).