KAOD(Monoamine Oxidase) CAS 9001-66-5
Identification
CAS Number
9001-66-5
Name
Monoamine Oxidase
EC Number
1.5.3 (Recombinant from microorganism)
Molecular Formula
C7H15N2O6P
Molecular Weight
55 kDa (SDS-PAGE)
EINECS
232-623-0
Safety Data
WGK Germany
3
Specifications and Other Information of Our Monoamine Oxidase CAS 9001-66-5
| Specification | Standards |
| Appearance | Yellow amorphous powder, lyophilized |
| Protein purity | ≥90% (from SDS-PAGE) |
| Activity | ≥4 U/mg solid |
| Catalase | ≤0.001% |
| ATPase | ≤0.005% |
| Glucose oxidase | ≤0.03% |
| Cholesterol oxidase | ≤0.003% |
General Assay Characteristics
| Method | Enzymatic/RATE A/E |
| Reagents | Ready to use |
| Calibrators | Level 1-0.0 U/L; Level 1-50 U/L |
| Calibration Stability | 28 days |
| Sample Type | EDTA,SST,Serum,Heparinised plasma |
| Assay Range | 0-200U/L undiluted |
| Standardisation |
Assay Principle
The assay is based on the increase in absorbance at 555 nm as the formation of quinoneimine dye proceeds in the forward reactions.
Unit Definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of H2O2 per min under the conditions described below.
Proposed MAO Recipe
Reagent I
| Composition | Concentration | Catalog Number |
| Tris-HCl buffer | 50mM | |
| NADH | 0.45mM | CS001 |
| α-Ketoglutarate | 15mM | |
| Detergent, Preservative |
Reagent II
| Composition | Concentration | Catalog Number |
| Tris-HCl buffer | 50mM | |
| Benzylamine | 10mM | |
| Detergent,Preservative |
Major reagent ingredients made by Assay Specilist Lab are list on right
Standard&Control
Home Made: MAO in buffer
Reaction curve chart
Known Application
The enzyme is useful for the determination of fructosyl-L-amino acid.
