Human&Mouse Chimeric WT-1 (HLA-A*02:01) Tetramer Protein, Accession: A0A140T913

  • Overview
  • Specifications
  • Shipping, Reconstitution & Storage
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  • Overview

    General Description

    Recombinant Human&Mouse Chimeric WT-1 (HLA-A*02:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr206(Human HLA-A*02:01 α1&α2)&Asp207-Ala362(Mouse HLA-A*02:01 α3), Ile21-Met119 (B2M) and RMFPNAPYL peptide.

    Biotinylated

    Specifications

    Target

    WT-1 (HLA-A*02:01) Tetramer

    Synonyms

    MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33

    Accession

    A0A140T913

    Species

    Human&Mouse

    Exact Sequence

    Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Ala362(Mouse HLA-A*02:01 α3), Ile21-Met119(B2M)and RMFPNAPYL peptide

    Molecular Weight

    The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

    Activity

    Immobilized Human&Mouse Chimeric WT-1 (HLA-A*02:01) Tetramer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 11.8ng/ml determined by ELISA. See testing image for detail.

    Product Tag

    C-His-Avi

    Express System

    HEK293

    Purity

    > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

    Endotoxin

    Less than 1EU per ug by the LAL method.

    Shipping, Reconstitution & Storage

    Appearance

    Liquid

    Shipping Conditions

    Shipped with blue ice.

    Formulation

    Supplied as 0.22um filtered solution in PBS (pH 7.4).

    Reconstitution

    Stability & Storage

    Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles.

    This product is branded by ChemWhat and sold through our website and here is the corresponding link ChemWhat Official Website.

    This product is sold exclusively through our sales company Watson International Limited (Ltd), and please click the link below for details. (https://www.watson-int.com/), and here is the corresponding link https://www.watson-int.com/Human&Mouse-Chimeric-WT-1-(HLA-A*02:01)-Tetramer-Protein-accession-A0A140T913/.